Details

Autor(en) / Beteiligte

• Mohanta, Uday Kumar
• Chikufenji, Boniface
• Galon, Eloiza May
• Ji, Shengwei
• Ma, Zhuowei
• El-Sayed, Shimaa Abd El-Salam
• Amer, Moaz M.
• Do, Thanh Thom
• Xuan, Xuenan

Titel

Molecular characterization and phylogeny of Anaplasma marginale, A. phagocytophilum and A. bovis in livestock of Bangladesh

Ist Teil von

• Parasitology international, 2023-12, Vol.97, p.102790-102790, Article 102790

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

ScienceDirect

Beschreibungen/Notizen

• The emergence of Tick-borne Anaplasma spp. poses a significant threat to humans and animals worldwide. Traditional surveys based on examining blood smears overlook the existence of emerging pathogens. This study aimed to screen Anaplasma spp. in livestock species from diverse geographies with molecular tools. We collected 276 blood samples from cattle (Bos indicus), gayals (Bos frontalis) and goats (Capra hircus) in Jhenaidah, Bogura, Sirajganj and Bandarban districts, and Naikhongchari sub-district from June 2021 to March 2022. After that, a molecular screening was conducted through polymerase chain reaction (PCR) and sequencing was done to confirm the PCR results. The PCR assays were performed based on the analyses of groEL (Anaplasma marginale) and 16S rRNA (A. phagocytophilum and A. bovis). The Anaplasma spp. detected in this study were A. marginale (10.51%), A. phagocytophilum (0.72%), and A. bovis (63.77%). However, A. platys was not detected in this study. Among the screened pathogens, the detection of A. bovis (82.86%) was significantly high in the Bandarban district, while A. marginale was found only in cattle in this location. Regarding animal species, the occurrence of A. bovis was significantly higher in cattle. Moreover, the detection rate of A. marginale was significantly higher in adult cattle (≥2 years). The phylogenetic analyses revealed that the groEL sequences of A. marginale and 16S rRNA sequences of A. bovis and A. phagocytophilum were included in a single clade in the respective phylograms, showing a single genotype of each species circulating in Bangladesh. This study reports the existence of A. phagocytophilum in Bangladesh for the first time. [Display omitted] •This study reports Anaplasma phagocytophilum in Bangladesh for the first time.•This is the first report of Anaplasma spp. in Jhenaidah, Bogura and Bandarban.•Our study reveals a geographic separation of A. marginale and A. phagocytophilum.•This is the first molecular report on Anaplasma spp. in gayals in Bangladesh.