Details

Autor(en) / Beteiligte

• Hwang, Tsong-Long
• Chang, Chuan-Hsin

Titel

Oridonin enhances cytotoxic activity of natural killer cells against lung cancer

Ist Teil von

• International immunopharmacology, 2023-09, Vol.122, p.110669-110669, Article 110669

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Oridonin extracted from Isodon rubescens is an active diterpene possessing anti-inflammatory and cancer-fighting properties.•Oridonin enhances the cytotoxic effects of natural killer (NK) cells against lung cancer cells by modulating degranulation markers, cytokines, the expression of surface receptors on NK cells, and the expression of receptor ligands in lung cancer cells.•Oridonin inhibits the phosphorylation of STAT3 in lung cancer cells and NK cells.•Oridonin has a synergistic effect on the in vivo anti-tumor activity of NK cells. Oridonin is a Chinese herbal medicine exhibiting anti-tumor properties; however, its immune modulation capacity has yet to be elucidated. Our objective in this study was to determine whether oridonin enhances the anti-tumor activity of natural killer (NK) cells against lung cancer cells. LDH-releasing assays were used to investigate the effects of oridonin on NK-92MI cell activity against lung cancer cells. Flow cytometry and real-time PCR were used to examine the effects of oridonin on degranulation markers, cytotoxic factors, activating receptors on NK-92MI cells, and ligands in lung cancer cells. Western blot analysis provided insight into the mechanisms underlying the observed effects. Oridonin enhanced the cytotoxic effects of NK-92MI cells against A549 lung cancer cells. This effect involved upregulating the expression of the degranulation marker CD107a and IFN-γ as well as activating receptors on NK cells and their ligand MICA/B. Oridonin also inhibited STAT3 phosphorylation in A549 cells and NK-92MI cells. A lung cancer mouse model confirmed the anti-tumor effects of oridonin and NK-92MI cells, wherein both treatments alone suppressed tumor growth. Oridonin was also shown to have a synergistic effect on the anti-tumor activity of NK-92MI cells. The ability of oridonin to enhance the cytotoxic effects of NK cells indicates its potential as a novel therapeutic agent for the treatment of lung cancer.