Details

Autor(en) / Beteiligte

• Battistini, Alessio
• Capitanio, Daniele
• Bailo, Paolo
• Moriggi, Manuela
• Tambuzzi, Stefano
• Gelfi, Cecilia
• Piccinini, Andrea

Titel

Proteomic analysis by mass spectrometry of postmortem muscle protein degradation for PMI estimation: A pilot study

Ist Teil von

• Forensic science international, 2023-08, Vol.349, p.111774-111774, Article 111774

Ort / Verlag

Ireland: Elsevier B.V

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The determination of the postmortem interval is a topic of great forensic interest. The possibility of using new technologies has allowed the study of postmortem decay of biomolecules in the determination of PMI. Skeletal muscle proteins are promising candidates because skeletal muscle exhibits slower postmortem decay compared to other internal organs and nervous tissues, while its degradation is faster than cartilage and bone. In this pilot study, skeletal muscle tissue from pigs was degraded at two different controlled temperatures, 21 °C and 6 °C, and analysed at predefined times points: 0, 24, 48, 72, 96, and 120 h. The obtained samples were analysed by mass spectrometry proteomics approach for qualitative and quantitative evaluation of proteins and peptides. Immunoblotting validation was performed for the candidate proteins. The results obtained appeared significant and identified several proteins useful for possible postmortem interval estimation. Of these proteins, PDLIM7, TPM1, and ATP2A2 were validated by immunoblotting at a larger number of experimental points and at different temperatures. The results obtained are in agreement with those observed in similar works. In addition, the use of a mass spectrometry approach increased the number of protein species identified, providing a larger panel of proteins for PMI assessment. •PMI assessment is crucial in forensic pathology.•Proteomic approach seems to be helpful in PMI estimation.•Mass spectrometry represents an important advance in the analyses of protein markers.