Details

Autor(en) / Beteiligte

• Venetz, Jessica
• Żygadłowska, Olga M.
• Lenstra, Wytze K.
• Helmond, Niels A. G. M.
• Nuijten, Guylaine H. L.
• Wallenius, Anna J.
• Dalcin Martins, Paula
• Slomp, Caroline P.
• Jetten, Mike S. M.
• Veraart, Annelies J.

Titel

Versatile methanotrophs form an active methane biofilter in the oxycline of a seasonally stratified coastal basin

Ist Teil von

• Environmental microbiology, 2023-11, Vol.25 (11), p.2277-2288

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2023

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The potential and drivers of microbial methane removal in the water column of seasonally stratified coastal ecosystems and the importance of the methanotrophic community composition for ecosystem functioning are not well explored. Here, we combined depth profiles of oxygen and methane with 16S rRNA gene amplicon sequencing, metagenomics and methane oxidation rates at discrete depths in a stratified coastal marine system (Lake Grevelingen, The Netherlands). Three amplicon sequence variants (ASVs) belonging to different genera of aerobic Methylomonadaceae and the corresponding three methanotrophic metagenome‐assembled genomes (MOB‐MAGs) were retrieved by 16S rRNA sequencing and metagenomic analysis, respectively. The abundances of the different methanotrophic ASVs and MOB‐MAGs peaked at different depths along the methane oxygen counter‐gradient and the MOB‐MAGs show a quite diverse genomic potential regarding oxygen metabolism, partial denitrification and sulphur metabolism. Moreover, potential aerobic methane oxidation rates indicated high methanotrophic activity throughout the methane oxygen counter‐gradient, even at depths with low in situ methane or oxygen concentration. This suggests that niche‐partitioning with high genomic versatility of the present Methylomonadaceae might contribute to the functional resilience of the methanotrophic community and ultimately the efficiency of methane removal in the stratified water column of a marine basin.