Details

Autor(en) / Beteiligte

• Beernink, Rik H.J.
• Schuitemaker, Joost H.N.
• Zwertbroek, Eva F.
• Scherjon, Sicco A.
• Cremers, Thomas I.F.H.

Titel

Early pregnancy biomarker discovery study for spontaneous preterm birth

Ist Teil von

• Placenta (Eastbourne), 2023-08, Vol.139, p.112-119

Ort / Verlag

Netherlands: Elsevier Ltd

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• discover new candidate biomarkers for spontaneous preterm birth in early pregnancy samples. When fully clinically validated, early pregnancy biomarkers for sPTB give the possibility to intervene or monitor high-risk pregnancies more intensively through, as example, pelvic exams, ultrasound or sonographic cervical length surveillance. Early pregnancy serum samples of eight spontaneous extreme and very preterm birth cases (<32 weeks of gestational age) without any symptoms of preeclampsia and fetal growth restriction and eight uncomplicated pregnancies were analyzed by liquid chromatography mass spectrometry (LC-MS). Thirteen proteins, which were differentially expressed according to the LC-MS data, were subsequently selected for confirmation by enzyme-linked immunosorbent assay (ELISA). Differential expression of four candidate biomarkers was confirmed by ELISA with decreased early pregnancy levels of gelsolin and fibulin-1 and increased levels of c-reactive protein and complement C5 in the preterm birth group. The confirmed candidate biomarkers are all to some extent related to inflammatory pathways and/or the complement system. This supports the hypothesis that both play a role in extreme and very preterm birth without any symptoms of preeclampsia and fetal growth restriction. The predictive value of complement C5, c-reactive protein, fibulin-1 and gelsolin should, therefore, be validated in another cohort with early pregnancy samples. •This study is a biomarker discovery study for spontaneous preterm birth.•Differential first trimester expression of five candidate biomarkers was observed.•These markers were related to inflammations and the complement system.•This supports that both play a role in the development of spontaneous preterm birth.