Details

Autor(en) / Beteiligte

• Pereida-Aguilar, Juan Carlos
• Barragán-Vargas, Cecilia
• Domínguez-Sánchez, Carlos
• Álvarez-Martínez, Roberto Carlos
• Acevedo-Whitehouse, Karina

Titel

Bacterial dysbiosis and epithelial status of the California sea lion (Zalophus californianus) in the Gulf of California

Ist Teil von

• Infection, genetics and evolution, 2023-09, Vol.113, p.105474-105474, Article 105474

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Despite the high incidence of urogenital carcinoma (UGC) in California sea lions stranded along California, no UGC has been reported in other areas of their distribution; however, cell morphologies typical of premalignant states have been found. Risk factors for UGC include high of organochlorines and infection with a gammaherpesvirus, OtHV-1, but the importance of the bacteriome for epithelial status remains unknown. We characterized the genital bacteriome of adult female California sea lions along their distribution in the Gulf of California and examined whether the diversity and abundance of the bacteriome varied spatially, whether there were detectable differences in the bacteriome between healthy and altered epithelia, and whether the bacteriome was different in California sea lions infected with OtHV-1 or papillomavirus. We detected 2270 ASVs in the genital samples, of which 35 met the criteria for inclusion in the core bacteriome. Fusobacteriia and Clostridia were present in all samples, at high abundances, and Actinobacteria, Alphaproteobacteria, and Campylobacteria were also well-represented. Alpha diversity and abundance of the California sea lion genital bacteriome varied geographically. The abundance of bacterial ASVs varied depending on the genital epithelial status and inflammation, with differences driven by classes Fusobacteriia, Clostridia, Campylobacteria and Alphaproteobacteria. Alpha diversity and abundance were lowest in samples in which OtHV-1 was detected, and highest those with papillomavirus. Our study is the first investigation of how the bacteriome is related to epithelial status in a wild marine species prone to developing cancer. [Display omitted] •First high throughput sequencing of the genital bacteriome of adult female California sea lions.•2,270 bacterial ASVs in the genital samples; 35 met criteria for inclusion in the core bacteriome.•Diversity and abundance of bacteria of the genital epithelium of sea lions varies geographically.•The abundance of bacterial ASVs varied depending on the cytological status and inflammation of the genital epithelia.•Otarine gammaherpesvirus and papillomavirus were associated with differences in bacterial ASV abundance and diversity.