Details

Autor(en) / Beteiligte

• Wang, Min
• Osborn, Lucas J.
• Jain, Sunit
• Meng, Xiandong
• Weakley, Allison
• Yan, Jia
• Massey, William J.
• Varadharajan, Venkateshwari
• Horak, Anthony
• Banerjee, Rakhee
• Allende, Daniela S.
• Chan, E. Ricky
• Hajjar, Adeline M.
• Wang, Zeneng
• Dimas, Alejandra
• Zhao, Aishan
• Nagashima, Kazuki
• Cheng, Alice G.
• Higginbottom, Steven
• Hazen, Stanley L.
• Brown, J. Mark
• Fischbach, Michael A.

Titel

Strain dropouts reveal interactions that govern the metabolic output of the gut microbiome

Ist Teil von

• Cell, 2023-06, Vol.186 (13), p.2839-2852.e21

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The gut microbiome is complex, raising questions about the role of individual strains in the community. Here, we address this question by constructing variants of a complex defined community in which we eliminate strains that occupy the bile acid 7α-dehydroxylation niche. Omitting Clostridium scindens (Cs) and Clostridium hylemonae (Ch) eliminates secondary bile acid production and reshapes the community in a highly specific manner: eight strains change in relative abundance by >100-fold. In single-strain dropout communities, Cs and Ch reach the same relative abundance and dehydroxylate bile acids to a similar extent. However, Clostridium sporogenes increases >1,000-fold in the ΔCs but not ΔCh dropout, reshaping the pool of microbiome-derived phenylalanine metabolites. Thus, strains that are functionally redundant within a niche can have widely varying impacts outside the niche, and a strain swap can ripple through the community in an unpredictable manner, resulting in a large impact on an unrelated community-level phenotype. [Display omitted] •Construction of variants of a complex community missing two strains in a metabolic niche•In the ΔCsΔCh community, eight strains change in relative abundance by >100-fold•Clostridium sporogenes increases >1,000-fold in the ΔCs but not ΔCh dropout•A strain swap can ripple through the community in an unpredictable manner Construction of variants of a complex defined microbial community reveals that strains that are functionally redundant within a niche can have widely varying impacts outside the niche.