Details

Autor(en) / Beteiligte

• Khatami, Fatemeh
• Shahriari, Shadab
• Aminimoghaddam, Soheila
• Klashami, Zeynab Nickhah
• Farahani, Maryam Shahrabi
• Teimoori-Toolabi, Ladan
• Amoli, Mahsa M
• Asadi, Mojgan
• Rashidi, Batool Hossein

Titel

PTEN promoter methylation and expression in endometrial cancer tissues

Ist Teil von

• Epigenomics, 2023-04, Vol.15 (8), p.507-516

Ort / Verlag

England

Erscheinungsjahr

2023

Beschreibungen/Notizen

• Some gene expression regulation in cancers can be controlled by epigenetic change like methylation.  promoter methylation and expression were evaluated in endometrial cancer. The study was run on 39 tumor tissues of endometrial cancer patients and 41 normal endometrial tissues. After total RNA extraction, cDNA synthesis was done by reverse transcription of the total (real-time PCR) using SYBER Green master mix. DNA extraction and bisulfite treatment were conducted and methylation was semiquantified by the methylation-sensitive high-resolution melting method. Finally, promoter methylation quantification of the total number of 25 tumors and 22 non-neoplastic tissues was done. gene expression showed a significant decrease in endometrial cancer tissues. Promoter methylation was significantly lower in the non-neoplastic group (7.2; p < 0.001). In addition, promoter methylation was observed in 52.0% of tumor tissues compared with 13.6% in the non-neoplastic group (p = 0.06). There were no significant correlations between expression and methylation and clinicopathological features in endometrial cancer patients (p > 0.05). gene expression in endometrial cancer tissues decreased because of its promoter hypermethylation.