Details

Autor(en) / Beteiligte

• Leitao, S M
• Navikas, V
• Miljkovic, H
• Drake, B
• Marion, S
• Pistoletti Blanchet, G
• Chen, K
• Mayer, S F
• Keyser, U F
• Kuhn, A
• Fantner, G E
• Radenovic, A

Titel

Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds

Ist Teil von

• Nature nanotechnology, 2023-09, Vol.18 (9), p.1078-1084

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Springer Nature - Connect here FIRST to enable access

Beschreibungen/Notizen

• In current nanopore-based label-free single-molecule sensing technologies, stochastic processes influence the selection of translocating molecule, translocation rate and translocation velocity. As a result, single-molecule translocations are challenging to control both spatially and temporally. Here we present a method using a glass nanopore mounted on a three-dimensional nanopositioner to spatially select molecules, deterministically tethered on a glass surface, for controlled translocations. By controlling the distance between the nanopore and glass surface, we can actively select the region of interest on the molecule and scan it a controlled number of times and at a controlled velocity. Decreasing the velocity and averaging thousands of consecutive readings of the same molecule increases the signal-to-noise ratio by two orders of magnitude compared with free translocations. We demonstrate the method's versatility by assessing DNA-protein complexes, DNA rulers and DNA gaps, achieving down to single-nucleotide gap detection.