Details

Autor(en) / Beteiligte

• Wang, Xu
• Edwards, Rachel L.
• Ball, Haley S.
• Heidel, Kenneth M.
• Brothers, Robert C.
• Johnson, Claire
• Haymond, Amanda
• Girma, Misgina
• Dailey, Allyson
• Roma, Jose Santinni
• Boshoff, Helena I.
• Osbourn, Damon M.
• Meyers, Marvin J.
• Couch, Robin D.
• Odom John, Audrey R.
• Dowd, Cynthia S.

Titel

MEPicides: α,β-unsaturated Fosmidomycin N‑Acyl Analogs as Efficient Inhibitors of Plasmodium falciparum 1‑Deoxy‑d‑xylulose-5-phosphate reductoisomerase

Ist Teil von

• ACS infectious diseases, 2023-07, Vol.9 (7), p.1387-1395

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Malaria, a mosquito-borne disease caused by several parasites of the Plasmodium genus, remains a huge threat to global public health. There are an estimated 0.5 million malaria deaths each year, mostly among African children. Unlike humans, Plasmodium parasites and a number of important pathogenic bacteria employ the methyl erythritol phosphate (MEP) pathway for isoprenoid synthesis. Thus, the MEP pathway represents a promising set of drug targets for antimalarial and antibacterial compounds. Here, we present new unsaturated MEPicide inhibitors of 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR), the second enzyme of the MEP pathway. A number of these compounds have demonstrated robust inhibition of Plasmodium falciparum DXR, potent antiparasitic activity, and low cytotoxicity against HepG2 cells. Parasites treated with active compounds are rescued by isopentenyl pyrophosphate, the product of the MEP pathway. With higher levels of DXR substrate, parasites acquire resistance to active compounds. These results further confirm the on-target inhibition of DXR in parasites by the inhibitors. Stability in mouse liver microsomes is high for the phosphonate salts, but remains a challenge for the prodrugs. Taken together, the potent activity and on-target mechanism of action of this series further validate DXR as an antimalarial drug target and the α,β-unsaturation moiety as an important structural component.