Details

Autor(en) / Beteiligte

• Yancey, Colleen E.
• Kiledal, E. Anders
• Chaganti, Subba Rao
• Denef, Vincent J.
• Errera, Reagan M.
• Evans, Jacob T.
• Hart, Lauren N.
• Isailovic, Dragan
• James, William S.
• Kharbush, Jenan J.
• Kimbrel, Jeffrey A.
• Li, Wei
• Mayali, Xavier
• Nitschky, Helena
• Polik, Catherine A.
• Powers, McKenzie A.
• Premathilaka, Sanduni H.
• Rappuhn, Nicole A.
• Reitz, Laura A.
• Rivera, Sara R.
• Zwiers, Claire C.
• Dick, Gregory J.

Titel

The Western Lake Erie culture collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome

Ist Teil von

• Harmful algae, 2023-07, Vol.126, p.102440, Article 102440

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• •Twenty-one xenic Microcystis cultures were isolated from western Lake Erie and capture the diversity of Microcystis strains observed in natural populations as well as their associated bacteria.•Microcystis strains within the culture collection show variability in core and accessory gene content, capture much of the strain diversity observed in the 2014 cyanoHAB, and genetically similar strains produce varying concentrations and congeners of microcystins.•This collection is a valuable resource for studying strain diversity and interactions between Microcystis and associated bacteria.•Our collection increases the availability of environmentally relevant strains from temperate North America, which is historically underrepresented in culture collections. Cyanobacterial harmful algal blooms (cyanoHABs) dominated by Microcystis spp. have significant public health and economic implications in freshwater bodies around the world. These blooms are capable of producing a variety of cyanotoxins, including microcystins, that affect fishing and tourism industries, human and environmental health, and access to drinking water. In this study, we isolated and sequenced the genomes of 21 primarily unialgal Microcystis cultures collected from western Lake Erie between 2017 and 2019. While some cultures isolated in different years have a high degree of genetic similarity (genomic Average Nucleotide Identity >99%), genomic data show that these cultures also represent much of the breadth of known Microcystis diversity in natural populations. Only five isolates contained all the genes required for microcystin biosynthesis while two isolates contained a previously described partial mcy operon. Microcystin production within cultures was also assessed using Enzyme-Linked Immunosorbent Assay (ELISA) and supported genomic results with high concentrations (up to 900 μg L⁻¹) in cultures with complete mcy operons and no or low toxin detected otherwise. These xenic cultures also contained a substantial diversity of bacteria associated with Microcystis, which has become increasingly recognized as an essential component of cyanoHAB community dynamics. These results highlight the genomic diversity among Microcystis strains and associated bacteria in Lake Erie, and their potential impacts on bloom development, toxin production, and toxin degradation. This culture collection significantly increases the availability of environmentally relevant Microcystis strains from temperate North America. [Display omitted]