Details

Autor(en) / Beteiligte

• Chen, Hao-Ran
• Su, Ming-Li
• Lei, Yan-Mei
• Ye, Zhuo-Xin
• Chen, Zhao-Peng
• Ma, Pin-Yi
• Yuan, Ruo
• Zhuo, Ying
• Yang, Chao-Yong
• Liang, Wen-Bin

Titel

Insights of Life Molecules’ Dynamic Distribution in Live Cells via Sequence–Structure Bispecific Fluorescent RNA

Ist Teil von

• Journal of the American Chemical Society, 2023-06, Vol.145 (23), p.12812-12822

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Life molecules’ distributions in live systems construct the complex dynamic reaction networks, whereas it is still challenging to demonstrate the dynamic distributions of biomolecules in live systems. Herein, we proposed a dynamic analysis strategy via sequence-structure bispecific RNA with state-adjustable molecules to monitor the dynamic concentration and spatiotemporal localization of these biomolecules in live cells based on the new insight of fluorescent RNA (FLRNA) interactions and their mechanism of fluorescence enhancement. Typically, computer-based nucleic acid-molecular docking simulation and molecular theoretical calculation have been proposed to provide a simple and straightforward method for guiding the custom-design of FLRNA. Impressively, a novel FLRNA with sequence and structure bispecific RNA named as a structure-switching aptamer (SSA) was introduced to monitor the real-time concentration and spatiotemporal localization of biomolecules, contributing to a deeper insight of the dynamic monitoring and visualization of biomolecules in live systems.