Details

Autor(en) / Beteiligte

• Feitoza, Luiz Henrique Maciel
• de Carvalho, Luis Paulo Costa
• da Silva, Lucas Rosendo
• Meireles, Anne Caroline Alves
• Rios, Flávia Geovana Fontineles
• Silva, Geisiane Santos
• de Paulo, Paula Frassinetti Medeiros
• Pessoa, Felipe Arley Costa
• de Medeiros, Jansen Fernandes
• Julião, Genimar Rebouças

Titel

Influence of meteorological and seasonal parameters on the activity of Culicoides paraensis (Diptera: Ceratopogonidae), an annoying anthropophilic biting midge and putative vector of Oropouche Virus in Rondônia, Brazilian Amazon

Ist Teil von

• Acta tropica, 2023-07, Vol.243, p.106928-106928, Article 106928

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• •7315 individuals of C. paraensis were captured.•Temperature, moisture, and seasonality affected the midge abundance.•No sample was positive for Oropouche Virus.•This study was the first to perform an entomovirological surveillance for OROV in the amazonian state of Rondônia. Biting midges of the genus Culicoides are insects of proven medical and veterinary importance, because of their role in the transmission of viruses, bacteria, protozoa and nematodes. Culicoides paraensis has been considered the main vector of the Oropouche Virus (OROV) in the urban cycle of the disease in the neotropics. Due to the great abundance of Culicoides spp. in the State of Rondônia and its epidemiological history of OROV, we investigated the biting activity in humans, the abundance as a function of meteorological parameters and seasonality, and the detection of OROV. Entomological collections occurred in three municipalities from Brazilian State of Rondônia: Porto Velho, Ariquemes, and Ouro Preto do Oeste. GLMM's were used to determine if Culicoides spp. abundance was predicted by seasonal, diurnal, and meteorological factors. Total RNA was extracted from insects and viral RNA detection was performed using the S segment as the target region of OROV via RT-qPCR. In total, 7315 individuals were captured and identified as C. paraensis. In the dry season, 1488 individuals (24.5%) were recorded, 4591 (75.5%) in the rainy season, with peaks of biting activity between 4pm and 6pm. All variables showed a significative effect on the midge abundance. The rainy season, temperature between 30 °C and 32 °C and relative air humidity between 75% and 85% were the main predictive parameters for capturing the highest average number of insects. Our results confirm diurnal activity of C. paraensis and its greatest abundance in rainy periods. No sample was positive for the OROV, which could be explained by the virus absence in local human populations, C. paraensis as a minor vector species in the sampled localities, and probable low rate of infection of biting midges. Our findings on hourly and seasonal biting activities can provide support to intervention actions regarding vector control and surveillance of this species. This was the first study to collect and analyze biting midges in a region where human OROV cases had already been detected, but without previous information on entomovirological surveillance. Our study aimed to investigate the main vector of Oropouche Virus - Culicoides paraensis, a frequent and abundant insect in the urban settings of the Brazilian Amazon. [Display omitted]