Details

Autor(en) / Beteiligte

• Kolmer, J. A.
• Bajgain, P.
• Rouse, M. N.
• Li, J.
• Zhang, P.

Titel

Mapping and characterization of the recessive leaf rust resistance gene Lr83 on wheat chromosome arm 1DS

Ist Teil von

• Theoretical and applied genetics, 2023-05, Vol.136 (5), p.115-115, Article 115

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Key message The leaf rust resistance gene in Thatcher wheat derivative 78–1 was mapped to chromosome 1DS with SNP markers and designated as Lr83 . ‘ Thatcher’ wheat near isogenic line RL6149, a putative derivative of Triticum dicoccoides , was previously determined to carry leaf rust resistance gene Lr64 on chromosome arm 6AL and a second gene temporarily named LrX on chromosome arm 1DS. The objective of this study was to map and characterize LrX in a population of recombinant inbred lines (RILs) that segregated for a single gene. Thatcher line 78–1 with LrX was crossed with Thatcher and individual F 2 seedlings and F 6 RILs were evaluated for leaf rust response. The 208 F 2 plants segregated for a single recessive gene and 148 F 6 lines for a single gene. The RILs and parents were characterized by genotyping by sequencing (GBS). Six GBS markers and five Kompetitive Allele-Specific PCR (KASP) markers were used to map LrX on the distal region of chromosome arm 1DS. LrX was 1 centiMorgan (cM) proximal to marker K-IWB38437 and 0.4 cM distal to GBS marker 1D_9037138 . Line 78–1 was crossed with Thatcher wheat lines with Lr21 , Lr42 , and Lr60 for allelism tests. LrX mapped 19.49 cM from Lr21 and 11.93 cM from Lr42 . In the cross of line 78–1 with the Thatcher line with Lr60 , one recombinant in 1,003 F 2 plants was found. LrX and Lr60 are at tightly linked loci on the distal region of chromosome arm 1DS. The gene in line 78–1 was designated as Lr83 . Cytological examination of RL6149 provided no evidence of transfer of a chromosome segment of an A- or B-genome chromosome to chromosome 1D.