Details

Autor(en) / Beteiligte

• Mani, Kannan
• Vitenberg, Tzach
• Ben-Mordechai, Lilach
• Schweitzer, Ron
• Opatovsky, Itai

Titel

Comparative untargeted metabolic analysis of natural- and laboratory-reared larvae of black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae)

Ist Teil von

• Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2023-06, Vol.266, p.110851-110851, Article 110851

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• In the present study, we examined the metabolic composition of black soldier fly (BSF) larvae from natural populations (Ruhama: R and She'ar Yashuv: S) and from a laboratory-reared colony (C) using untargeted metabolomics analysis. The results revealed significant over-accumulation of metabolites from phenylalanine and purine metabolism and biosynthesis of phenylalanine, tyrosine and tryptophan, and arginine in both natural populations, and enriched pathway analysis, compared to the laboratory-reared colony. In addition, we found accumulation of glutathione metabolism and aminoacyl tRNA biosynthesis related metabolites in R, and linoleic acid and tryptophan metabolism related metabolites in S. Moreover, we found down-accumulation of metabolites belonging to alanine, aspartate and glutamate metabolism in both natural populations: amino sugar and nucleotide sugar metabolism only in the R population and aminoacyl-tRNA biosynthesis, glyoxylate and dicarboxylate metabolism only in the S population. Overall, the results suggest that the naturally growing larvae require large quantities of metabolites from aromatic amino acids (phenylalanine, tyrosine and tryptophan) for defense against pathogens under natural conditions e.g., melanization. In addition, glutathione metabolites help the BSF to survive under oxidative stress. Further study of the functional metabolomics of naturally growing and laboratory-reared larvae could provide a platform for better understanding of BSF larval survival mechanisms in complex environments. [Display omitted] •BSF larvae lack biosynthesis genes for aromatic amino acids (AAA) and B vitamins•Metabolites from AAA metabolism and biosynthesis over-accumulated in natural larvae•Metabolites from alanine, aspartate and glutamate metabolism were down-accumulated•This down-accumulation indicates metabolic disturbance or nutrient deficiency.•Presence of AAA and B vitamin metabolites implies they originate in diet or symbionts.