Details

Autor(en) / Beteiligte

• Liu, Xue-Qing
• Yan, Xiao-Hui
• Liang, Jun
• Kuang, Hai-Xue
• Xia, Yong-Gang

Titel

Microwave assisted free radical degradation of Schisandra polysaccharides: Optimization, identification and application

Ist Teil von

• International journal of biological macromolecules, 2023-05, Vol.237, p.124107-124107, Article 124107

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• In order to establish structural-fingerprinting of polysaccharides for improvement of quality assessment, a sample preparation method based on microwave assisted free radical degradation (MFRD) of plant polysaccharides was proposed to produce oligosaccharides and small Mw polysaccharides. As a case study of Schisandra chinensis and S. sphenanthera fruit polysaccharides (SCP and SSP), the MFRD condition (i.e., 100 °C, 30 s and 80 W) was confirmed to be optimal. The potential structures of the MFRD products of SCP and SSP were further discussed by combinations of HILIC-ESI−-QTOF-MSE and HILIC-ESI−-Q-OT-IT-MS/MS. As followed, multivariable statistical analysis shows a clear separation of SCP and the SSP in PCA and OPLS-DA plots based HILIC-ESI−-QTOF-MSE data. The VIP plot unveils several key Q-markers (e.g., peaks 3, 8, 9, 10, 15, 25, 26, 28, 29 and 30) with significant differences and stable emergences. Furthermore, a low-polymerization compositional fingerprinting was successfully constructed for SCP and SSP using a high-performance anion-exchange chromatography with pulsed amperometric detection. Compared to the conventional sample preparation methods, the MFRD took only a few thousandth of the time to accomplish degradations of plant polysaccharides. It significantly improves sample preparations and is generally applicable to various polysaccharide samples. •A sample preparation method based MFRD of SCP and SSP was proposed for the first time.•The potential structures of the MFRD products of SCP and SSP were discussed.•Structural-fingerprinting of SCP and SSP was built using HILIC-ESI−-QTOF-MSE and HPAEC-PAD.