Details

Autor(en) / Beteiligte

• Won, TaeJun
• Mohid, Sk Abdul
• Choi, JiHye
• Kim, MinSoo
• Krishnamoorthy, Janarthanan
• Biswas, Indranil
• Bhunia, Anirban
• Lee, DongKuk

Titel

The role of hydrophobic patches of de novo designed MSI-78 and VG16KRKP antimicrobial peptides on fragmenting model bilayer membranes

Ist Teil von

• Biophysical chemistry, 2023-05, Vol.296, p.106981-106981, Article 106981

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Antimicrobial peptides (AMPs) with cell membrane lysing capability are considered potential candidates for the development of the next generation of antibiotics. Designing novel AMPs requires an in-depth understanding of the mechanism of action of the peptides. In this work, we used various biophysical techniques including 31P solid-state NMR to examine the interaction of model membranes with amphipathic de novo-designed peptides. Two such peptides, MSI-78 and VG16KRKP, were designed with different hydrophobicity and positive charges. The model lipid membranes were constituted by mixing lipids of varying degrees of ‘area per lipid’ (APL), which directly affected the packing properties of the membrane. The observed emergence of the isotropic peak in 31P NMR spectra as a function of time is a consequence of the fragmentation of the membrane mediated by the peptide interaction. The factors such as the charges, overall hydrophilicity of the AMPs, as well as lipid membrane packing, contributed to the kinetics of membrane fragmentation. Furthermore, we anticipate the designed AMPs follow the carpet and toroidal pore mechanisms when lysing the cell membrane. This study highlights the significance of the effect of the overall charges and the hydrophobicity of the novel AMPs designed for antimicrobial activity. [Display omitted] •Difference in lipid-peptide interaction is studied using three antimicrobial peptides (AMPs) with different amphipathicity•The area per lipid (APL) of the membrane plays an important role in the interaction of AMPs with different model membranes•Fragmentation of lipid membrane packing by AMPs are dependent on the overall charges and hydrophobicity of AMPs.