Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
MAEL facilitates metabolic reprogramming and breast cancer progression by promoting the degradation of citrate synthase and fumarate hydratase via chaperone‐mediated autophagy
Ist Teil von
The FEBS journal, 2023-07, Vol.290 (14), p.3614-3628
Ort / Verlag
England: Blackwell Publishing Ltd
Erscheinungsjahr
2023
Quelle
Wiley Online Library
Beschreibungen/Notizen
Metabolic reprogramming is a hallmark of cancer. Several studies have shown that inactivation of Krebs cycle enzymes, such as citrate synthase (CS) and fumarate hydratase (FH), facilitates aerobic glycolysis and cancer progression. MAEL has been shown to play an oncogenic role in bladder, liver, colon, and gastric cancers, but its role in breast cancer and metabolism is still unknown. Here, we demonstrated that MAEL promoted malignant behaviours and aerobic glycolysis in breast cancer cells. Mechanistically, MAEL interacted with CS/FH and HSAP8 via its MAEL domain and HMG domain, respectively, and then enhanced the binding affinity of CS/FH with HSPA8, facilitating the transport of CS/FH to the lysosome for degradation. MAEL‐induced degradation of CS and FH could be suppressed by the lysosome inhibitors leupeptin and NH4Cl, but not by the macroautophagy inhibitor 3‐MA or the proteasome inhibitor MG132. These results suggested that MAEL promoted the degradation of CS and FH via chaperone‐mediated autophagy (CMA). Further studies showed that the expression of MAEL was significantly and negatively correlated with CS and FH in breast cancer. Moreover, overexpression of CS or/and FH could reverse the oncogenic effects of MAEL. Taken together, MAEL promotes a metabolic shift from oxidative phosphorylation to glycolysis by inducing CMA‐dependent degradation of CS and FH, thereby promoting breast cancer progression. These findings have elucidated a novel molecular mechanism of MAEL in cancer.
Metabolic reprogramming is a hallmark of cancer. We demonstrate that MAEL interacts with the Krebs cycle enzymes citrate synthase (CS) and fumarate hydratase (FH) and the chaperone HSAP8 via its MAEL domain and HMG domain, respectively, and then increases the binding affinity of CS/FH with HSPA8, facilitating transport of CS/FH to the lysosome for degradation, thereby leading to a metabolic switch to glycolysis and carcinogenesis.