Details

Autor(en) / Beteiligte

• Young Chung, Jee
• Thone, Melissa N.
• Davies, Jenny E.
• Gach, Johannes S.
• Huw Davies, D.
• Forthal, Donald N.
• Kwon, Young Jik

Titel

Vaccination against SARS-CoV-2 using extracellular blebs derived from spike protein-expressing dendritic cells

Ist Teil von

• Cellular immunology, 2023-04, Vol.386, p.104691-104691, Article 104691

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• •Cell-free, cell-mimicking platform tested for COVID-19 vaccination.•Presentation of SARS-CoV-2 spike (S) protein by DC-derived extracellular blebs (EBs)•Efficient antibody production and virus neutralization by S-presenting, DC-derived EBs. COVID-19 has caused significant morbidity and mortality worldwide but also accelerated the clinical use of emerging vaccine formulations. To address the current shortcomings in the prevention and treatment of SARS-CoV-2 infection, this study developed a novel vaccine platform that closely mimics dendritic cells (DCs) in antigen presentation and T-cell stimulation in a cell-free and tunable manner. Genetically engineered DCs that express the SARS-CoV-2 spike protein (S) were chemically converted into extracellular blebs (EBs). The resulting EBs elicited potentially protective humoral immunity in vivo, indicated by the production of antibodies that potently neutralized S-pseudotyped virus, presenting EBs as a promising and safe vaccine.