Details

Autor(en) / Beteiligte

• Jett, Julianne D.
• Beck, Rachael
• Tyutyunnyk, Diana
• Sanchez, Jesus
• Lopez‐Cruzan, Marisa
• Ginsburg, Brett C.
• McPherson, Sterling M.
• Javors, Martin A.
• McDonell, Michael G.
• Hill‐Kapturczak, Nathalie

Titel

Validation of the quantification of phosphatidylethanol 16:0/18:1 concentrations in TASSO‐M20 devices

Ist Teil von

• Alcohol, clinical & experimental research, 2023-04, Vol.47 (4), p.748-755

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Background Phosphatidylethanol 16:0/18:1 (PEth), found in whole blood, is a biomarker for alcohol consumption with high sensitivity, specificity, and a long detection window. The TASSO‐M20 device is used to self‐collect capillary blood from the upper arm and has advantages over finger stick methods. The purpose of this study was to (1) validate PEth measurement using the TASSO‐M20 device, (2) describe the TASSO‐M20 for blood self‐collection during a virtual intervention, and (3) characterize PEth, urinary ethyl glucuronide (uEtG) and self‐reported alcohol in a single participant over time. Methods PEth levels in blood samples dried on TASSO‐M20 plugs were compared to those in (1) liquid whole blood (N = 14) and (2) dried blood spot cards (DBS; N = 23). Additionally, the self‐reported drinking, positive or negative uEtG results (dip card cutoff ≥300 ng/mL), and observed self‐collection of blood with TASSO‐M20 devices for PEth levels were obtained over time during virtual interviews of a single contingency management participant. High‐performance liquid chromatography with tandem mass spectrometry detection was used to measure PEth levels for both preparations. Results PEth concentrations from dried blood on TASSO‐M20 plugs and liquid whole blood were correlated (0 to 1700 ng/mL; N = 14; r2 = 0.988; slope = 0.951) and in a subgroup of samples with lower concentrations (N = 7; 0 to 200 ng/mL; r2 = 0.944, slope = 0.816). PEth concentrations from dried blood on TASSO‐M20 plugs and DBS were correlated (0 to 2200 ng/mL; N = 23; r2 = 0.927; slope = 0.667) and in a subgroup of samples with lower concentrations (N = 16; 0 to 180 ng/mL; r2 = 0.978, slope = 0.749). Results of the contingency management participant indicate that changes in PEth levels (TASSO‐M20) and uEtG concentrations were consistent with each other and with changes in self‐reported alcohol use. Conclusions Our data support the utility, accuracy, and feasibility of using the TASSO‐M20 device for blood self‐collection during a virtual study. The TASSO‐M20 device had multiple advantages over the typical finger stick method, including consistent blood collection, participant acceptability, and less discomfort as indicated by acceptability interviews. This study validated methods for assessing phosphatidylethanol 16:0/18:1 (PEth) levels following blood collection with the TASSO‐M20 device. PEth levels on TASSO‐M20 plugs were compared to the standard methods of liquid whole blood or dried blood spot (DBS). PEth concentrations on TASSO‐M20 plugs were highly correlated with liquid whole blood (0–200 ng/mL; r2 = 0.944, slope = 0.816) and DBS (0–180 ng/mL; r2 = 0.978, slope = 0.749). The TASSO‐M20 device is an accurate blood collection method for PEth quantification.