Details

Autor(en) / Beteiligte

• Peng, Lihui
• Matellan, Carlos
• Bosch‐Fortea, Minerva
• Gonzalez‐Molina, Jordi
• Frigerio, Matteo
• Salentinig, Stefan
• del Rio Hernandez, Armando
• Gautrot, Julien E.

Titel

Mesenchymal Stem Cells Sense the Toughness of Nanomaterials and Interfaces

Ist Teil von

• Advanced healthcare materials, 2023-05, Vol.12 (13), p.e2203297-n/a

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Stem cells are known to sense and respond to the mechanical properties of biomaterials. In turn, cells exert forces on their environment that can lead to striking changes in shape, size and contraction of associated tissues, and may result in mechanical disruption and functional failure. However, no study has so far correlated stem cell phenotype and biomaterials toughness. Indeed, disentangling toughness‐mediated cell response from other mechanosensing processes has remained elusive as it is particularly challenging to uncouple Youngs' or shear moduli from toughness, within a range relevant to cell‐generated forces. In this report, it is shown how the design of the macromolecular architecture of polymer nanosheets regulates interfacial toughness, independently of interfacial shear storage modulus, and how this controls the expansion of mesenchymal stem cells at liquid interfaces. The viscoelasticity and toughness of poly(l‐lysine) nanosheets assembled at liquid‐liquid interfaces is characterised via interfacial shear rheology. The local (microscale) mechanics of nanosheets are characterised via magnetic tweezer‐assisted interfacial microrheology and the thickness of these assemblies is determined from in situ ellipsometry. Finally, the response of mesenchymal stem cells to adhesion and culture at corresponding interfaces is investigated via immunostaining and confocal microscopy. Mesenchymal stem cells sense the toughness of polymer nanosheets assembled at liquid–liquid interfaces. The self‐assembly of poly(l‐lysine) and pentafluorobenzoyl chloride results in nanomaterials with interfacial toughness varying over orders of magnitudes. Stem cells spreading at these interfaces can fracture brittle interfaces but not those with the highest toughness, equivalent to that of steel.