Details

Autor(en) / Beteiligte

• Chen, Lei
• Sha, Ming-Lei
• Chen, Fei-Teng
• Jiang, Chen-Yi
• Li, Deng
• Xu, Chao-Liang
• Pan, De-Shen
• Xu, Zi-Jie
• Tang, Qi-Lin
• Xia, Shu-Jie
• Sun, Lian-Hui
• Fan, Guang-Jian
• Shao, Yi

Titel

Upregulation of KLF14 expression attenuates kidney fibrosis by inducing PPARα-mediated fatty acid oxidation

Ist Teil von

• Free radical biology & medicine, 2023-02, Vol.195, p.132-144

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Tubulointerstitial fibrosis (TIF) is essential during the development of end-stage kidney disease (ESKD) and is associated with the impairment of fatty acid oxidation (FAO). Kruppel-like factor 14 (KLF14) is an important gene in lipid metabolism, but its role in TIF remains unknown. TGF-β-stimulated HK-2 cells and mouse unilateral ureteral obstruction (UUO) were used as renal fibrosis models. The role of KLF14 in the process of renal fibrosis was verified by gene knockout mice, genetic or pharmacological interference in animal model and cell model respectively. In the current study, we found that KLF14 expression increased after activation of the TGF-β signaling pathway during TIF. In KLF14−/− mice, more severe fibrosis was observed after unilateral ureteral obstruction (UUO) was induced. In human HK2 cells, knockdown of KLF14 led to more severe fibrosis induced by TGF-β1, while overexpression of KLF14 partially attenuated this process. Specifically, KLF14 deficiency decreased mitochondrial FAO activity, resulting in lipid accumulation. Thus, the energy supply to the cells was insufficient, finally resulting in TIF. We further proved that KLF14 could target peroxisome proliferator activated receptor alpha (PPARα) as a transcriptional activator. This study identified the upregulation of KLF14 expression in response to kidney stress during the process of fibrosis. Upon TIF, the activated TGF-β signaling pathway can enhance KLF14 expression, while the upregulation of KLF14 expression can decrease the degree of TIF by improving FAO activity in tubular epithelial cells and recovering the energy supply mediated by PPARα. [Display omitted] •Upregulation of KLF14 expression in response to kidney stress during the process of fibrosis.•Upon tubulointerstitial fibrosis, the activated TGF-β signaling pathway can enhance KLF14 expression.•KLF14 can decrease the degree of tubulointerstitial fibrosis by improving fatty acid oxidation activity in tubular epithelial cells.•Klf14 plays a role in improving fatty acid oxidation through transcriptional regulation of PPARα.