Details

Autor(en) / Beteiligte

• Xu, Fei
• Jiang, Meixia
• Tang, Qin
• Lin, Jiaqi
• Liu, Xun
• Zhang, Chuanli
• Zhao, Jinzhi
• He, Yanjin
• Dong, Lijie
• Zhu, Limin
• Lin, Tingting

Titel

MiR-29a-3p inhibits high-grade transformation and epithelial–mesenchymal transition of lacrimal gland adenoid cystic carcinoma by targeting Quaking

Ist Teil von

• Molecular biology reports, 2023-03, Vol.50 (3), p.2305-2316

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Lacrimal adenoid cystic carcinoma (LACC) is the most common orbital malignant epithelial neoplasm. LACC with high-grade transformation (LACC-HGT) has higher rates of recurrence, metastasis, and mortality than LACC without HGT. This study investigated the effects of microRNA-29a-3p (miR-29a-3p) in the pathogenesis of LACC-HGT. Methods An Agilent human miRNA microarray was used to screen the differentially expressed miRNAs (DEMs) in LACC and LACC-HGT tumor tissues. Then, the primary cells obtained in previous studies were used to determine the effect of miR-29a-3p. Results The expression of miR-29a-3p was abnormally lower in LACC-HGT than in LACC. miR-29a-3p can specifically target the 3ʹ UTR of Quaking mRNA and down-regulate Quaking expression, thereby inhibiting the proliferation, migration, and epithelial–mesenchymal transition of LACC cells. Conclusions This study illustrated that miR-29a-3p functions as a tumor suppressor by down-regulating the expression of Quaking to inhibit the tumorigenesis of LACC cells. This study may also reveal the pathogenesis of HGT in LACC cells and provide a reference for LACC-HGT targeted diagnosis.