Details

Autor(en) / Beteiligte

• Tian, Huan
• Liu, Shuai‐Qi
• Jing, Wen‐Hui
• Hao, Zhi‐Hua
• Li, Ying‐Hui
• Lu, Zhe‐Hao
• Ding, Ze‐Kai
• Huang, Shao‐Li
• Xu, Yu‐Song
• Wang, Hua‐Bing

Titel

Imaginal disc growth factor is involved in melanin synthesis and energy metabolism in Bombyx mori

Ist Teil von

• Archives of insect biochemistry and physiology, 2023-04, Vol.112 (4), p.e21995-n/a

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The imaginal disc growth factor (IDGF), belonging to the glycoside hydrolase 18 family, plays an important role in various physiological processes in insects. However, the detail physiological function of IDGF is still unclear. In this study, transcriptome analysis was performed on the fatbody isolated from staged control and BmIDGF mutant silkworm larvae. Transcriptional profiling revealed that the absence of BmIDGF significantly affected differentially expressed genes involved in tyrosine and purine metabolism, as well as multiple energy metabolism pathways, including glycolysis, galactose, starch, and sucrose metabolism. The interruption of BmIDGF caused similar and specific gene expression changes to male and female fatbody. Furthermore, a genome‐scale metabolic network integrating metabolomic and transcriptomic datasets revealed 11 pathways significantly altered at the transcriptional and metabolic levels, including amino acid, carbohydrate, uric acid metabolism pathways, insect hormone biosynthesis, and ABC transporters. In conclusion, this multiomics analysis suggests that IDGF is involved in gene‐metabolism interactions, revealing its unique role in melanin synthesis and energy metabolism. This study provides new insights into the physiological function of IDGF in insects. In this study, transcriptome analysis was performed on the fatbody isolated from staged control and BmIDGF mutants silkworm larvae. Transcriptional profiling revealed that the absence of BmIDGF significantly affected differentially expressed genes involved in tyrosine and purine metabolism, as well as multiple energy metabolism pathways. Furthermore, a genome‐scale metabolic network integrating metabolomic and transcriptomic datasets revealed that IDGF is involved in gene‐metabolism interactions, revealing its unique role in melanin synthesis and energy metabolism. Highlights The interruption of BmIDGF significantly affected tyrosine and purine metabolism, as well as multiple energy metabolism pathways. The absence of BmIDGF caused similar and specific gene expression changes to male and female fatbody. IDGF plays an essential role in melanin synthesis and energy metabolism.