Details

Autor(en) / Beteiligte

• Diaz Perez, Alda
• Pysz, Patrick M.
• Usdrowski, Hunter
• Hunter, Victoria K.
• Stenken, Julie A.

Titel

Attachment and optimization of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa biofilms to a 3D printed lattice

Ist Teil von

• Journal of microbiological methods, 2023-01, Vol.204, p.106644-106644, Article 106644

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• A lattice was designed and fabricated using three-dimensional (3D) printing that allows for the facile transfer of biofilms formed from either Staphylococcus aureus, Staphylococcus epidermidis, or Pseudomonas aeruginosa into a fresh cell culture flask. To enhance biofilm production onto the filaments, three protein-based treatments were compared: fetal bovine serum (FBS), bovine serum albumin (BSA), and fibrinogen (Fb). Protein treatments included either supplementing the growth broths or pre-coating the lattice prior to immersion into the broth. S. aureus and P. aeruginosa biofilms were observed on all tested filaments that contained the supplement Fb. S. epidermidis required BSA to form biofilm. Ultimately, polycarbonate (PC) was chosen as the optimal material for lattice creation since it can be autoclaved without warping key design features. In addition, this 3D printed design may facilitate biofilm transfer from the bacterial culture to different cell culture platforms. •Three-dimensional 3D printed lattice allows facile biofilm transfer.•Polycarbonate 3D printed lattice was autoclavable and maintained features.•3D printed lattice was used with P. aeruginosa, S.aureus and S. epidermidis.•Optimized protein treatments promoted biofilm growth on to the 3D printing lattice.