Details

Autor(en) / Beteiligte

• Ruccolo, Serge
• Brito, Gilmar
• Christensen, Melodie
• Itoh, Tetsuji
• Mattern, Keith
• Stone, Kevin
• Strotman, Neil A.
• Sun, Alexandra C.

Titel

Electrochemical Recycling of Adenosine Triphosphate in Biocatalytic Reaction Cascades

Ist Teil von

• Journal of the American Chemical Society, 2022-12, Vol.144 (49), p.22582-22588

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2022

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Adenosine triphosphate (ATP) provides the driving force necessary for critical biological functions in all living organisms. In synthetic biocatalytic reactions, this cofactor is recycled in situ using high-energy stoichiometric reagents, an approach that generates waste and poses challenges with enzyme stability. On the other hand, an electrochemical recycling system would use electrons as a convenient source of energy. We report a method that uses electricity to turn over enzymes for ATP generation in a simplified cellular respiration mimic. The method is simple, robust, and scalable, as well as broadly applicable to complex enzymatic processes including a four-enzyme biocatalytic cascade in the synthesis of the antiviral molnupiravir.