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Electrochemical Recycling of Adenosine Triphosphate in Biocatalytic Reaction Cascades
Ist Teil von
Journal of the American Chemical Society, 2022-12, Vol.144 (49), p.22582-22588
Ort / Verlag
United States: American Chemical Society
Erscheinungsjahr
2022
Quelle
MEDLINE
Beschreibungen/Notizen
Adenosine triphosphate (ATP) provides the driving force necessary for critical biological functions in all living organisms. In synthetic biocatalytic reactions, this cofactor is recycled in situ using high-energy stoichiometric reagents, an approach that generates waste and poses challenges with enzyme stability. On the other hand, an electrochemical recycling system would use electrons as a convenient source of energy. We report a method that uses electricity to turn over enzymes for ATP generation in a simplified cellular respiration mimic. The method is simple, robust, and scalable, as well as broadly applicable to complex enzymatic processes including a four-enzyme biocatalytic cascade in the synthesis of the antiviral molnupiravir.