Details

Autor(en) / Beteiligte

• Liu, Wenpeng
• Lee, Luke P.

Titel

Toward Rapid and Accurate Molecular Diagnostics at Home

Ist Teil von

• Advanced materials (Weinheim), 2023-05, Vol.35 (21), p.e2206525-n/a

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• The global outbreaks of infectious diseases have significantly driven an imperative demand for rapid and accurate molecular diagnostics. Nucleic acid amplification tests (NAATs) feature high sensitivity and high specificity; however, the labor‐intensive sample preparation and nucleic acid amplification steps remain challenging in order to carry out rapid and precision molecular diagnostics at home. This review discusses the advances and challenges of automatic solutions of sample preparation integrated with on‐chip nucleic acid amplification for effective and accurate molecular diagnostics at home. The sample preparation methods of whole blood, urine, saliva/nasal swab, and stool on chip are examined. Then, the repurposable integrated sample preparation on a chip using various biological samples is investigated. Finally, the on‐chip NAATs that can be integrated with automated sample preparation are evaluated. The user‐friendly approaches with combined sample preparation and NAATs can be the game changers for next‐generation rapid and precision home diagnostics. Recent advancements in integrated sample preparations and nucleic acid (NA) detection are reported for rapid and accurate home diagnostics. Automated sample preparation methods of blood, urine, nasal swabs, saliva, or stool that can be integrated with NA amplification on a single device are highlighted. Recent progress, challenges, and perspectives of user‐friendly integrated molecular diagnostics at home are discussed.