Details

Autor(en) / Beteiligte

• Huang, Zhi‐Xiong
• He, Xiao‐Rong
• Ding, Xin‐Yu
• Chen, Jia‐Hao
• Lei, Yi‐Hong
• Bai, Jian‐Bing
• Lin, Dian‐Chao
• Hong, Yi‐Huang
• Lan, Jian‐Fa
• Chen, Qiong‐Hua

Titel

Lipoxin A4 depresses inflammation and promotes autophagy via AhR/mTOR/AKT pathway to suppress endometriosis

Ist Teil von

• American journal of reproductive immunology (1989), 2023-03, Vol.89 (3), p.e13659-n/a

Ort / Verlag

Denmark: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Background Endometriosis is a benign gynecological disease with the feature of estrogen dependence and inflammation. The function of autophagy and the correlation with inflammation were not yet revealed. Methods Autophagosomes were detected by transmission electron microscopy. Gene Expression Omnibus (GEO) database was referred to analyze the expression of autophagy‐related genes. Quantification of mRNA and protein expression was examined by qRT‐PCR and Western Blot. Immunohistochemistry was performed to explore the expression of proteins in tissues. The mouse model of endometriosis was performed to analyze the autophagic activity and effect of LXA4. Results The expression of autophagy‐related genes in endometriotic lesions were unusually changed. The number of autophagosomes and LC3B‐II expression was diminished, and p62 was increased in ectopic lesions from both patients and mice. Interleukin 1β (IL1β) attenuated the expression of LC3B and promoted the level p62. The autophagy activator MG‐132 upregulated the expression of LC3B and reduced IL1β, IL6, and p62. LXA4 reversed the inhibitory effect of IL1β on the expression of LC3B and p62, and blocking the receptor of LXA4 AhR (aryl hydrocarbon receptor) resulted in the incapacitation of LXA4 to influence the effect of IL1β. LXA4 depressed the phosphorylation of AKT and mTOR to against IL1β, and blocking AhR negatively regulated the effect of LXA4 on AKT/mTOR pathway. LXA4 reduced the ectopic lesions and the expression of IL1β and p62, but enhanced LC3B‐II in endometriotic mouse models. Conclusion In endometriosis, increased inflammation of ectopic lesions prominently depresses autophagy. LXA4 could regulate autophagy by suppressing inflammatory response through AhR/AKT/mTOR pathway.