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Taxonomic and genomic characterization of Sporosarcina cyprini sp. nov., moderately tolerant of Cr+6 and Cd+2 isolated from the gut of invasive fish Cyprinus carpio var. communis (Linn., 1758)
Ist Teil von
Antonie van Leeuwenhoek, 2023-03, Vol.116 (3), p.193-206
Ort / Verlag
Cham: Springer International Publishing
Erscheinungsjahr
2023
Link zum Volltext
Quelle
SpringerLink (Online service)
Beschreibungen/Notizen
A Gram-stain-positive, motile, and rod-shaped bacterium, designated as strain MB25
T
, was isolated from the gut of
Cyprinus carpio
from the highly polluted river Yamuna, India. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MB25
T
belonged to the genus
Sporosarcina
, sharing the highest sequence similarity with
S. luteola
Y1
T
(98.98%) and
S. koreensis
S-K12
T
(98.91%). Digital DNA-DNA hybridization and average nucleotide identity values of strain MB25
T
with strain Y1
T
and S-K12
T
were 18.9, 77.69, and 18.2, 76.80 respectively. Genome analysis of strain MB25
T
revealed its biotechnological properties such as tolerance to potent heavy metals, genes for the production of carbohydrate-active enzymes, antimicrobial compounds, and also degradation of aromatic compounds. The G + C content of strain MB25
T
genome was 45%. Growth observed at 10–40 °C (optimum, 28–30 °C), pH 6.0–8.5 (optimum pH 7.5–8.0); NaCl concentrations up to 6.0% (w/v). The dominant respiratory quinone was MK-7, cell wall peptidoglycan is of the A-4 type containing amino acids Lys-Glu and the major fatty acids are anteiso-C
11:0
and iso-C
15: 0
. The major polar lipids of strain MB25
T
are diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of phenotypic, chemotaxonomic, phylogenetic, and phylogenomic data, strain MB25
T
represents a novel species of the genus
Sporosarcina,
for which the name
Sporosarcina cyprini
sp. nov. is proposed. The type strain is MB25
T
(= MCC 4366
T
= JCM 34521
T
= CCM 9113
T
).