Details

Autor(en) / Beteiligte

• Guo, Rui
• Wang, Si
• Niu, Nan‐Nan
• Xu, Ya‐Li
• Zhu, Jun‐Xun
• Scheer, Hugo
• Noy, Dror
• Zhao, Kai‐Hong

Titel

Dichromic Allophycocyanin Trimer Covering a Broad Spectral Range (550–660 nm)

Ist Teil von

• Chemistry : a European journal, 2023-02, Vol.29 (9), p.e202203367-n/a

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Phycobilisomes, the light‐harvesting complexes of cyanobacteria and red algae, are a resource for photosynthetic, photonic and fluorescence labeling elements. They cover an exceptionally broad spectral range, but the complex superstructure and assembly have been an obstacle. By replacing in Synechocystis sp. PCC 6803 the biliverdin reductases, we studied the role of chromophores in the assembly of the phycobilisome core. Introduction of the green‐absorbing phycoerythrobilin instead of the red‐absorbing phycocyanobilin inhibited aggregation. A novel, trimeric allophycocyanin (Dic‐APC) was obtained. In the small (110 kDa) unit, the two chromophores, phycoerythrobilin and phytochromobilin, cover a wide spectral range (550 to 660 nm). Due to efficient energy transfer, it provides an efficient artificial light‐harvesting element. Dic‐APC was generated in vitro by using the contained core‐linker, LC, for template‐assisted purification and assembly. Labeling the linker provides a method for targeting Dic‐APC. When exploring the role of chromophores in phycobilisome assembly, a novel dichromic allophycocyanin was obtained that greatly extends the spectral range for light‐harvesting. A linker contained in the structure is successfully used for template‐assisted assembly in vitro. This approach would also allow for targeting, in artificial photosynthetic systems or fluorescence labeling.