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Details

Autor(en) / Beteiligte
Titel
Identification of Babesia bovis MSA-1 functionally constraint regions capable of binding to bovine erythrocytes
Ist Teil von
  • Veterinary parasitology, 2022-12, Vol.312, p.109834-109834, Article 109834
Ort / Verlag
Elsevier B.V
Erscheinungsjahr
2022
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
  • Merozoite surface antigen-1 is a glycoprotein expressed by Babesia bovis and is considered a vaccine candidate given that antibodies against it are able to partially block in vitro invasion of bovine erythrocytes. Despite this, no study to date has confirmed the target cell binding properties of the full MSA-1 or its fragments. This research has thus been focused on identifying protein regions playing a role in erythrocyte attachment, based on genetic diversity and natural selection analysis. Two regions under functional constraint (nucleotides 134–428 and 464–629) having a preponderance of negatively-selected signals were identified in silico. Three non-overlapping peptides derived from functionally constraint regions (42422 (39PEGSFYDDMSKFYGAVGSFD58), 42424 (91NALIKNNPMIRPDLFNATIV110) and 42426 (150TDIVEEDREKAVEYFKKHVY169)) were able to specifically bind to a sialoglycoprotein located on the bovine erythrocyte surface as confirmed by sensitive and specific peptide-cell interaction competition assays using both enzymatically treated and untreated red blood cells. Interestingly, it was predicted that peptides 42422 and 42426 have a helical structure and conserved motifs in all strain/isolates. These findings provide evidence, for the first time, related to B. bovis MSA-1 short regions used by the parasite in erythrocyte binding which could be predicted using natural selection analysis. Future work focused on evaluating these peptides’ antigenic ability during natural infection, and their ability to induce protection in immunisation assays are needed to confirm their usefulness as synthetic vaccine candidates. •Two functionally constraint regions were identified in B. bovis MSA-1.•B. bovis MSA-1 binds to bovine erythrocytes through 3 short protein fragments.•These MSA-1 peptides might be promising components of a B. bovis vaccine
Sprache
Englisch
Identifikatoren
ISSN: 0304-4017
eISSN: 1873-2550
DOI: 10.1016/j.vetpar.2022.109834
Titel-ID: cdi_proquest_miscellaneous_2734166232

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