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Absorption of iron from Tegillarca granosa using an in vitro simulated digestion and Caco‐2/HepG2 co‐culture system
Journal of the science of food and agriculture, 2023-01, Vol.103 (2), p.891-899
Sun, Bolun
Zhang, Panxue
Zhang, Jinjie
Huang, Tao
Li, Chao
Yang, Wenge
2023
Details
Autor(en) / Beteiligte
Sun, Bolun
Zhang, Panxue
Zhang, Jinjie
Huang, Tao
Li, Chao
Yang, Wenge
Titel
Absorption of iron from Tegillarca granosa using an in vitro simulated digestion and Caco‐2/HepG2 co‐culture system
Ist Teil von
Journal of the science of food and agriculture, 2023-01, Vol.103 (2), p.891-899
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2023
Link zum Volltext
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
Background Iron‐deficiency anemia is one severe micronutrient malnutrition and has captured worldwide attention. This study evaluated the in vitro iron absorption of two iron‐binding proteins (hemoglobin and ferritin) from Tegillarca granosa. In addition, the protein structure–iron absorption relationship and the regulatory effect of hepcidin on cellular iron absorption were explored. Results Our findings revealed that both hemoglobin and ferritin extracted from T. granosa contained abundant iron‐binding sites, as evidenced by stronger peaks in amide I and II regions compared with the two proteins from humans. Less β‐sheet (27.67%) structures were found in hemoglobin compared with ferritin (36.40%), probably contributing to its greater digestibility and more release of available iron. This was confirmed by the results of Caco‐2/HepG2 cell culture system that showed iron absorption of hemoglobin was 26.10–39.31% higher than that of ferritin with an iron content of 50–150 μmol L−1. This high iron absorption of hemoglobin (117.86–174.10 ng mg−1) could also be due to more hepcidin produced by HepG2 cells, thereby preventing ferroportin‐mediated iron efflux from Caco‐2 cells. In addition, the possible risk of oxidative stress was evaluated in cells post‐iron exposure. In comparison with ferrous sulfate, a common iron supplement, Caco‐2 cells treated with the iron‐binding proteins had a 9.50–25.73% lower level of intracellular reactive oxygen species, indicating the safety of hemoglobin and ferritin. Conclusion Collectively, the data of this research would be helpful for understanding the key features and potential of developing hemoglobin and ferritin from T. granosa as novel iron supplements. © 2022 Society of Chemical Industry.
Sprache
Englisch
Identifikatoren
ISSN: 0022-5142
eISSN: 1097-0010
DOI: 10.1002/jsfa.12200
Titel-ID: cdi_proquest_miscellaneous_2709744018
Format
–
Schlagworte
Absorption
,
Anemia
,
Animals
,
Arcidae
,
Binding sites
,
Caco-2 Cells
,
Caco‐2/HepG2 cell model
,
Cell culture
,
Coculture Techniques
,
Dietary supplements
,
Digestibility
,
Digestion
,
Efflux
,
Ferritin
,
Ferritins - metabolism
,
Ferrous sulfate
,
Hemoglobin
,
Hemoglobins
,
Hep G2 Cells
,
Hepcidin
,
Hepcidins - metabolism
,
Humans
,
Iron
,
Iron - metabolism
,
iron absorption
,
Iron deficiency
,
Iron sulfates
,
iron‐binding protein
,
Malnutrition
,
Nutrient deficiency
,
Oxidative stress
,
Protein structure
,
Proteins
,
Reactive oxygen species
,
Tegillarca granosa
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