Details

Autor(en) / Beteiligte

• Chong, Gang-Gang
• Ding, Liang-Yi
• Qiu, Yan-Yan
• Qian, Xiao-Long
• Dong, Ya-Li
• Li, Chun-Xiu
• Li, Aitao
• Pan, Jiang
• Xu, Jian-He

Titel

Building Flexible Escherichia coli Modules for Bifunctionalizing n‑Octanol: The Byproduct of Oleic Acid Biorefinery

Ist Teil von

• Journal of agricultural and food chemistry, 2022-08, Vol.70 (34), p.10543-10551

Ort / Verlag

American Chemical Society

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Artificial biorefinery of oleic acid into 1,10-decanedioic acid represents a revolutionizing route to the sustainable production of chemically difficult-to-make bifunctional chemicals. However, the carbon atom economy is extremely low (56%) due to the formation of unifunctional n-octanol. Here, we report a panel of recombinant Escherichia coli modules for diverse bifunctionalization, where the desired genetic parts are well distributed into different modules that can be flexibly combined in a plug-and-play manner. The designed ω-functionalizing modules could achieve ω-hydroxylation, consecutive ω-oxidation, or ω-amination of n-octanoic acid. By integrating these advanced modules with the reported oleic acid-cleaving modules, high-value C8 and C10 products, including ω-hydroxy acid, ω-amino acid, and α,ω-dicarboxylic acid, were produced with 100% carbon atom economy. These ω-functionalizing modules enabled the complete use of all of the carbon atoms from oleic acid (released from plant oil) for the green synthesis of structurally diverse bifunctional chemicals.