Details

Autor(en) / Beteiligte

• Huang, Wei-Sheng
• Wang, Li-Ting
• Chen, Jwo-Sheng
• Chen, Yen-Ting
• Wei, Sean Ting-Shyang
• Chiang, Yin-Ru
• Wang, Pei-Ling
• Lee, Tzong-Huei
• Lin, Shih-Ting
• Huang, Lina
• Shieh, Wung Yang

Titel

Vibrio salinus sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll in the western Pacific Ocean

Ist Teil von

• Antonie van Leeuwenhoek, 2022-09, Vol.115 (9), p.1203-1214

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2022

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• A marine, facultatively anaerobic, nitrogen-fixing bacterium, designated strain DNF-1 T , was isolated from the lagoon sediment of Dongsha Island, Taiwan. Cells grown in broth cultures were Gram-negative rods that were motile by means of monotrichous flagella. Cells grown on plate medium produced prosthecae and vesicle-like structures. NaCl was required and optimal growth occurred at about 2–3% NaCl, 25–30 °C and pH 7–8. The strain grew aerobically and was capable of anaerobic growth by fermenting D-glucose or other carbohydrates as substrate. Both the aerobic and anaerobic growth could be achieved with NH 4 Cl as a sole nitrogen source. When N 2 served as the sole nitrogen source only anaerobic growth was observed. Major cellular fatty acids were C 14:0 , C 16:0 and C 16:1 ω7c , while major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 42.2 mol% based on the genomic DNA data. Phylogenetic analyses based on 16S rRNA genes and the housekeeping genes, gapA , pyrH , recA and gyrB , revealed that the strain formed a distinct lineage at species level in the genus Vibrio of the family Vibrionaceae . These results and those from genomic, chemotaxonomic and physiological studies strongly support the assignment of a novel Vibrio species. The name Vibrio salinus sp. nov. is proposed for the novel species, with DNF-1 T (= BCRC 81209 T  = JCM 33626 T ) as the type strain. This newly proposed species represents the second example of the genus Vibrio that has been demonstrated to be capable of anaerobic growth by fixing N 2 as the sole nitrogen source.