Details

Autor(en) / Beteiligte

• Wang, Jennifer Y.
• Austin, Evan
• Jagdeo, Jared

Titel

Visible red light does not induce DNA damage in human dermal fibroblasts

Ist Teil von

• Journal of biophotonics, 2022-11, Vol.15 (11), p.e202200023-n/a

Ort / Verlag

Weinheim: WILEY‐VCH Verlag GmbH & Co. KGaA

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Visible red light (RL) therapy is a rapidly expanding treatment option for dermatological conditions, including acne, psoriasis and chronic wounds. It is currently unknown if high fluences of RL induce DNA damage via reactive oxygen species (ROS) stress or other pathways. Our lab previously demonstrated that RL generates ROS in human dermal fibroblasts (HDFs). Other studies show that UV and blue light generate ROS and DNA damage in fibroblasts. This study aims to determine if RL induces DNA damage in HDFs. We found that 320 J/cm2, 640 J/cm2 and 1280 J/cm2 RL (633 ± 6 nm) did not measurably increase DNA damage in the form of cyclobutane pyrimidine dimers (CPD) or 6‐4 photoproducts (6‐4PP) immediately, 3 hours and 24 hours following irradiation. Our study further supports that RL therapy is safe in human skin fibroblasts. Visible red light continues to gain adoption as an important therapeutic modality. This study demonstrates that visible red light does not cause DNA damage classically associated with ultraviolet light (CPDs or 6‐4PPs) in human skin fibroblasts in vitro. The determination that red light does not cause DNA damage adds to the already robust molecular and clinical safety profile of red light phototherapy.