Details

Autor(en) / Beteiligte

• Peng, Bi-Xia
• Li, Fangfang
• Mortimer, Monika
• Xiao, Xiang
• Ni, Ya
• Lei, Yuyang
• Li, Minjie
• Guo, Liang-Hong

Titel

Perfluorooctanoic acid alternatives hexafluoropropylene oxides exert male reproductive toxicity by disrupting blood-testis barrier

Ist Teil von

• The Science of the total environment, 2022-11, Vol.846, p.157313-157313, Article 157313

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• As alternatives to perfluorooctanoic acid (PFOA), hexafluoropropylene oxide (HFPO) homologues, including hexafluoropropylene oxide dimer acid (HFPO-DA), hexafluoropropylene oxide trimer acid (HFPO-TA), and hexafluoropropylene oxide tetramer acid (HFPO-TeA), have attracted widespread attention recently due to their environmental ubiquity and high potential for bioaccumulation and toxicity. In the present study, a set of in vivo mouse and in vitro mouse testicular Sertoli TM4 cell experiments were employed to explore the male reproductive toxicity and underlying mechanisms of HFPO homologues on blood-testis barrier. Tissue and permeability analyses of mice testes after 28-day treatment with 5 mg/kg/day HFPO-DA or PFOA, or 0.05 mg/kg/day HFPO-TA or HFPO-TeA indicated that there was an increase in the degradation of TJ protein occludin in mice with a disrupted blood-testis barrier (BTB). Following exposure to 100 μM HFPO-DA, HFPO-TA or 10 μM PFOA, HFPO-TeA, transepithelial electrical resistance measurements of TM4 cells also indicated BTB disruption. Additionally, as a result of the exposure, matrix metalloproteinase-9 expression was enhanced through activation of p38 MAPK, which promoted the degradation of occludin. On the whole, the results indicated HFPO homologues and PFOA induced BTB disruption through upregulation of p-p38/p38 MAPK/MMP-9 pathway, which promoted the degradation of TJ protein occludin and caused the disruption of TJ. [Display omitted] •Exposure to HFPO induced disruption of the blood-testis barrier on mice at human internal exposure levels;•HFPO disrupted the barrier through upregulation of p-p38/p38 MAPK/MMP-9 pathway and increased degradation of occludin;•Hexafluoropropylene oxide dimer acid and trimer acid exhibited stronger disruption effects than PFOA.