Details

Autor(en) / Beteiligte

• Tagliafierro, Teresa
• Joshi, Shreyas
• Sameroff, Stephen
• Marques, Adriana
• Dumler, J. Stephen
• Mishra, Nischay
• Sanchez-Vicente, Santiago
• Wormser, Gary P.
• Marcos, Luis A.
• Lipkin, W. Ian
• Tokarz, Rafal

Titel

Detection of antibodies to Anaplasma phagocytophilum and Babesia microti using linear peptides

Ist Teil von

• Ticks and tick-borne diseases, 2022-09, Vol.13 (5), p.101999-101999, Article 101999

Ort / Verlag

Elsevier GmbH

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •The incidence of infections with Anaplasma phagocytophilum and Babesia microti has sharply increased over the past two decades.•We identified and evaluated the utility of short linear peptides for serologic diagnosis of anaplasmosis and babesiosis using microarrays.•Our findings provide a foundation of testing the utility of these peptides in stand-alone serologic assays. Anaplasma phagocytophilum and Babesia microti are emerging tick-borne pathogens in the United States. Although active infection is typically diagnosed by direct diagnostic tests, such as blood smear or polymerase chain reaction assay, serologic assays can be helpful to identify past infections, and the use of acute plus convalescent testing can potentially identify recent infections. We employed a peptide array to select sets of linear peptides for serologic diagnosis of infections with A. phagocytophilum and B. microti. Three optimal peptides were selected for each agent based on their performance with clinical specimens. All three A. phagocytophilum peptides were located within the conserved fragments of the MSP2 antigen. Two B. microti peptides were located in the N terminus of the SA-1 antigen; the third was in the BMN 1-17 antigen. We found that these peptides can be a useful tool for detection of antibody reactivity to both of these pathogens.