Details

Autor(en) / Beteiligte

• Braga, Adelaide
• Gomes, Daniela
• Rainha, João
• Cardoso, Beatriz B.
• Amorim, Cláudia
• Silvério, Sara C.
• Fernández-Lobato, María
• Rodrigues, Joana L.
• Rodrigues, Lígia R.

Titel

Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4

Ist Teil von

• Applied microbiology and biotechnology, 2022-06, Vol.106 (12), p.4617-4626

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1- sacB that was able to produce 73.4 ± 1.6 g L −1 of FOS, with a productivity of 1.53 ± 0.03 g L −1  h −1 and a yield of 0.31 ± 0.03 g FOS g sucrose −1 . This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process. Key points • Zymomonas mobilis was engineered to produce FOS in a one-step fermentation process. • Mutant strains produced FOS mixtures with high concentration of 6-kestose. • A new route to produce tailor-made FOS mixtures was presented. Graphical abstract