Details

Autor(en) / Beteiligte

• Purohit, Megha K.
• Rathore, Dalip Singh
• Koladiya, Gopi
• Pandey, Sandeep
• Singh, Satya P.

Titel

Comparative analysis of the catalysis and stability of the native, recombinant and metagenomic alkaline proteases in organic solvents

Ist Teil von

• Environmental science and pollution research international, 2022-11, Vol.29 (53), p.80968-80982

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2022

Quelle

SpringerLink

Beschreibungen/Notizen

• The effect of organic solvents on alkaline proteases was assessed for native, recombinant, and metagenomically derived alkaline proteases. Their stability and the effects of physicochemical parameters were studied in the presence of hexane. The native enzyme was comparatively more resistant against the organic solvents than the recombinant counterparts. On the other hand, the metagenomically derived alkaline protease was minimally resistant against solvents. A similar trend was apparent for the stability of enzyme in organic solvents. The novelty of this study lies in the fact that the majority of the studies on the solvent tolerance have focused on the mesophilic enzymes, while those from the haloalkaliphilic bacteria have received little attention. The comparative tolerance of the native, recombinant, and metagenomic alkaline proteases against the organic solvent has practical importance. The phylogenetic relatedness among the various protease sequences will be described.