Details

Autor(en) / Beteiligte

• Yang, Si-Yu
• Huang, Meng-Xing
• Sun, Yan-Xia
• Li, Liang
• Bian, Zhen-Hua
• Long, Jie
• Zhao, Zhi-Bin

Titel

A 33-color panel of phenotypic analysis of murine organ specific immune cells

Ist Teil von

• Journal of immunological methods, 2022-08, Vol.507, p.113294-113294, Article 113294

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• This 33-color panel delineates immune phenotypes of the major lymphocyte subsets in murine tissues. The panel identifies surface markers for cell activation, differentiation, and exhaustion, and chemokine receptors, in CD4 T cells, CD8 T cells, γδ T cells, NK cells, NKT cells and B cells in spleen and liver. This panel was designed to include only surface markers to avoid the need for fixation and permeabilization steps. Cells were analyzed on a full spectrum flow cytometer, 5-Laser Aurora system (Cytek Biosciences). This panel enables in-depth immunophenotyping of murine lymphocytes in immune and non-immune tissues of mice. It could be a tool for systematic analysis of immune cell response to infectious diseases and immune disorders.