Details

Autor(en) / Beteiligte

• Hoog, Anna
• Villanueva-Hernández, Sonia
• Razavi, Mahsa Adib
• van Dongen, Katinka
• Eder, Thomas
• Piney, Lauriane
• Chapat, Ludivine
• de Luca, Karelle
• Grebien, Florian
• Mair, Kerstin H.
• Gerner, Wilhelm

Titel

Identification of CD4+ T cells with T follicular helper cell characteristics in the pig

Ist Teil von

• Developmental and comparative immunology, 2022-09, Vol.134, p.104462-104462, Article 104462

Ort / Verlag

United States: Elsevier Ltd

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• T follicular helper (Tfh) cells provide help to germinal center B cells for affinity maturation, class switch and memory formation. Despite these important functions, this subset has not been studied in detail in pigs due to a lack of species-specific antibodies. We investigated putative Tfh cells from lymphoid tissues and blood of healthy pigs by using cross-reactive antibodies for inducible T-cell costimulator (ICOS) and B-cell lymphoma 6 (Bcl-6). In lymph nodes, we identified a CD4+ T cell population with an ICOS+Bcl-6+CD8α+ phenotype, reminiscent of human and murine germinal center Tfh cells. Within blood-derived CD4+ T cells, sorted ICOShiCD25- and ICOSdimCD25dim cells were able to induce the differentiation of CD21+IgM+ B cells into Ig-secreting plasmablasts. Compared to naïve CD4+ T cells, these two phenotypes were 3- to 7-fold enriched for cells expressing the Tfh-related transcripts CD28, CD40LG, IL6R and MAF, as identified by single-cell RNA sequencing. These results provide a first characterization of Tfh cells in swine and confirm their ability to provide B-cell help. •CD4+ T cells with an ICOS+Bcl-6+CD8α+ phenotype form a unique subset in lymph nodes and spleen.•Foxp3-expressing ICOS+Bcl-6+ CD4+ T cells have a phenotype that is reminiscent of T follicular regulatory (Tfr) cells.•Blood-derived ICOShiCD25- and ICOSdimCD25dim CD4+ T cells can stimulate B cells for antibody production.