Details

Autor(en) / Beteiligte

• Ait Atmane, Sihem
• Ait Eldjoudi, Djedjiga
• Aksoylu Özbek, Zeynep
• Günç Ergönül, Pelin
• Khettal, Bachra

Titel

Acute and 28-day repeated dose toxicity evaluations of cold pressed Pinus halepensis Mill. seed oil in mice and rats

Ist Teil von

• Regulatory toxicology and pharmacology, 2022-07, Vol.132, p.105191-105191, Article 105191

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2022

Quelle

ScienceDirect Pay Per View(PPV) Titles

Beschreibungen/Notizen

• Pinus halepensis Mill. seed (Pinaceae), popularly known as ‘Zgougou’, is widely consumed in the Mediterranean countries and used traditionally in the treatment of some diseases such as bronchitis, rheumatism, infection, and inflammation. The present study aimed to evaluate the oral safety of cold pressed oil of Pinus halepensis Mill. seeds (COPHS) by acute and 28-day repeated dose toxicities studies in Wistar mice and rats, respectively. In the acute toxicity study, oral administration of COPHS to mice did not provoke mortality or any toxic signs at doses up to 5000 mg/kg bw. After administration of COPHS at doses of 250, 500, and 1000 mg/kg bw/day for 28 days, no abnormal changes were observed in body weight, water intake, food consumption, organ weight, blood haematological, serum biochemistry parameters, and histology profile. Furthermore, there was no animal death or any symptom of toxicity in any group during sub-acute toxicity test period. Our findings demonstrate that COPHS is relatively non-toxic and has a large safety margin (>5000 mg/kg). The results of the present research provide basic reference data for food consumption and for future in vivo screening of biological and pharmacological properties of cold pressed oil of Pinus halepensis Mill. seeds. [Display omitted] •The safety of cold pressed oil of Algerian Aleppo pine (Pinus halepensis Mill.) seed was examined.•LD50 of Pinus halepensis Mill. seed oil was >5000 mg/kg bw for mice oral administration.•Cold-pressed oil of Aleppo pine seed affected RBC, WBC, PLT, triglyceride, and urea.•Cold-pressed oil of Pinus halepensis Mill. seed can be considered a non-toxic source of essential fatty acids.