Details

Autor(en) / Beteiligte

• Bloesch, Zoë
• Nauheimer, Lars
• Elias Almeida, Thaís
• Crayn, Darren
• Field, Ashley Raymond

Titel

HybPhaser identifies hybrid evolution in Australian Thelypteridaceae

Ist Teil von

• Molecular phylogenetics and evolution, 2022-08, Vol.173, p.107526-107526, Article 107526

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Hybphaser, a new hybrid-detecting analytical approach for targeted capture sequence data, identified two hybrids in Australian Thelypterid ferns: Russell River Fern and Tully River Fern.•Hybphaser identified that parents for the Russell River Fern belonged to two different genera, making it the first genomically identified intergeneric hybrid in the family, and the first case of a fern for Australia.•Evidence that hybrid ancestry was common in a fern family suggests that hybrid-detecting analysis such as Hybphaser should become routine in investigation of targeted capture sequence datasets. Hybridisation has been proposed to play an important role in fern evolution, but has been difficult to investigate. This study explores the utility of target sequence capture and read-to-reference phasing of putative hybrids to investigate the role of evolutionary reticulation in ferns using Australian Thelypteridaceae as a model. The bioinformatics workflow HybPhaser was used to assess divergence between alleles, phase sequence reads to references and construct accessions resembling parental haplotypes. These accessions were included in phylogenetic and network analyses to detect hybrids and infer their parentage. This approach identified two novel hybrid lineages in Thelypteridaceae, one occurring between two different genera (Abacopteris and Christella), and another as part of a complex of Christella. In addition, hybrid phasing successfully reduced conflicting data and improved overall resolution in the Thelypteridaceae phylogeny, highlighting the power of this approach for reconstructing evolutionary history in reticulated lineages.