Details

Autor(en) / Beteiligte

• Yao, Jie
• Chen, Qian
• Zhu, Jun‐qi
• Cai, Rui‐gang

Titel

Targeted gene next‐generation sequencing reveals genomic profile in a cohort of 46 Chinese patients with breast cancer

Ist Teil von

• The journal of gene medicine, 2022-06, Vol.24 (6), p.e3420-n/a

Ort / Verlag

England: Wiley Periodicals Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Background The present study aimed to explore the genomic profile in a cohort of Chinese patients with breast cancer (BC), as well as provide potential strategies for clinic treatment in specific subset of BC patients. Methods Paired samples from 46 BC patients were subjected to DNA extraction and 537 gene targeted next‐generation sequencing. Results In total, 742 somatic mutations were detected in these patients, which involved 303 genes. TP53 and PIK3CA were the most frequently mutated genes, with a mutation rate of 45.65% and 26.09%. C>T, T>C and C>A comprised the main single nucleotide base variation for this Chinese cohort. Triple negative breast cancer (TNBC) group had more TP53‐mutated patients than the Non‐TNBC group (p = 0.0229). In addition, the cohort was also divided into ‘Young’ and ‘Old’ groups based on the age of onset. Compared with the ‘Young’ group, the ‘Old’ group had more frameshift mutations (p = 0.0190), less missense mutations (p = 0.0269) and more HOXA11‐mutated patients (p = 0.0197). Additionally, the HOXA11mt (HOXA11 gene mutated) group had more frameshift mutations than the HOXA11wt (HOXA11 gene without mutation) group (p < 0.0001). In KEGG (i.e. Kyoto Encyclopedia of Genes and Genomes) analysis, the HOXA11wt group had more gene mutations involved in the T cell receptor signaling pathway (p = 0.0197), Jak–STAT signaling pathway (p = 0.0380) and the HIF‐1 signaling pathway (p = 0.0489) than the HOXA11mt group. In the present study, the heterogeneity of somatic mutations was revealed between different tumor subgroups, including TNBC/Non‐TNBC, age of onset (Young/Old) and HOXA11 mutation (HOXA11mt/HOXA11wt). Conclusions The present study revealed the heterogeneity of gene mutation and clinical variables among BC subtypes and might provide guidance for developing a potential target for clinical treatment. Next‐generation sequencing of Chinese patients with breast cancer (BC) revealed the somatic gene variants, frequently mutated genes and the heterogeneity of somatic mutations between different hierarchical subgroups. These distinct mutation profiles might be potentially relevant in the development of treatment strategies for specific subset of BC patients.