Details

Autor(en) / Beteiligte

• Torres‐Martínez, R.
• Moreno‐León, A.
• García‐Rodríguez, Y.M.
• Hernández‐Delgado, T.
• Delgado‐Lamas, G.
• Espinosa‐García, F.J.

Titel

Tagetes lucida Cav. essential oil and the mixture of its main compounds are antibacterial and modulate antibiotic resistance in multi‐resistant pathogenic bacteria

Ist Teil von

• Letters in applied microbiology, 2022-08, Vol.75 (2), p.210-223

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2022

Quelle

Wiley Online Library

Beschreibungen/Notizen

• We evaluated an essential oil (EO) of Tagetes lucida Cav. and the mixture of its main compounds against multi‐drug resistant bacteria. We found that EO and the partially reconstituted blend of its main components have antibacterial activity and inhibit antibiotic resistance (ampicillin, chloramphenicol, nalidixic acid, vancomycin and imipenem) in strains of Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa HIM‐MR01. The T. lucida EO alone or added to the antibiotics showed antimicrobial activity against S. aureus and P. aeruginosa. The EO main bioactive compounds were methyl eugenol (relative abundance in EO: 46·15%), estragole (32·93%), linalool (2·48%) and geraniol (0·33%). The mixture (PREO) of those compounds at those proportions inhibited the growth of P. aeruginosa in 45% at 683·62 µg ml−1 and that of S. aureus in 51·7% at 39·04 µg ml−1. The PREO had higher antibacterial and modulatory activities than the original EO. In conclusion, we overcame the unpredictability of EO activity (due to their natural variability) by determining which EO components inhibited bacteria and then producing a PREO to generate a reproducible mixture with predictable antibacterial and modulation of resistance activities. Thus, the PREO, and its components, show potential as alternatives to manage multidrug‐resistant pathogens. Significance and Impact of the Study: To contribute to fighting antimicrobial resistance to antibiotics, we evaluated an essential oil (EO) of Tagetes lucida and the mixture of its main compounds (PREO) against multi‐drug resistant bacteria. The EO and the PREO have antibacterial activity and modulate antibiotic resistance in Staphylococcus aureus and Pseudomonas aeruginosa. Also, we overcame the natural unpredictability of the EOs activity by determining which EO components inhibited bacteria and then producing a consistent PREO with predictable antibacterial and modulatory antibiotic resistance activity. To our knowledge, this is the first time that this procedure has generated a reconstituted blend with predictable effects from EOs.