Food & function, 2022-05, Vol.13 (9), p.5115-5123
2022
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Bifidobacterium bifidum relieved DSS-induced colitis in mice potentially by activating the aryl hydrocarbon receptor
- Ist Teil von
- Food & function, 2022-05, Vol.13 (9), p.5115-5123
- Ort / Verlag
- England: Royal Society of Chemistry
- Erscheinungsjahr
- 2022
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Inflammatory bowel disease (IBD) characterized by relapsed intestinal inflammation and barrier function disruption is still a great therapeutic challenge. This study aimed to screen probiotics that have the potential to help alleviate IBD and further elucidate their mechanism of action. Caco-2 cell differentiated monolayers and RAW264.7 cells stimulated by lipopolysaccharide (LPS) were used for probiotic screening , and then the efficacies of the obtained six bacterial strains were evaluated in mice with dextran sulfate sodium (DSS)-induced colitis. The results showed that all of the strains at varying degrees could increase the transepithelial electrical resistance (TEER) value, decrease the influx of FITC-dextran in Caco-2 cell monolayers and attenuate the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in LPS-stimulated RAW264.7 cells. experiments indicated that FL-276.1 (FL-276.1) and FL-228.1 (FL-228.1) showed the best efficacies to ameliorate body weight loss, colon shortening, and intestinal barrier disruption. Accordingly, in FL-276.1 and FL-228.1 groups, the genes of zonula occludens-1 (ZO-1), claudin-4, occludin and mucin 2 (Muc2) in mouse colonic tissues were significantly upregulated, while TNF-α, IL-1β and IL-6 were downregulated. Further results showed that strains FL-276.1 and FL-228.1 could activate the aryl hydrocarbon receptor (AhR) in the intestine. Our study showed that the two strains, FL-276.1 and FL-228.1, ameliorated DSS-induced colitis by enhancing the intestinal barrier and anti-inflammation potentially the AhR pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2042-6496eISSN: 2042-650XDOI: 10.1039/d1fo04219jTitel-ID: cdi_proquest_miscellaneous_2649995785
- Format
- –
- Schlagworte
- Animal tissues, Animals, Aromatic compounds, Bifidobacterium bifidum, Bifidobacterium bifidum - metabolism, Body weight, Body weight loss, Caco-2 Cells, Cell differentiation, Colitis, Colitis - chemically induced, Colitis - genetics, Colitis - therapy, Colon, Colon - metabolism, Dextran, Dextran sulfate, Dextran Sulfate - adverse effects, Dextrans, Disease Models, Animal, Disruption, Electrical resistivity, Humans, Hydrocarbons, IL-1β, Inflammatory bowel disease, Inflammatory bowel diseases, Inflammatory Bowel Diseases - metabolism, Interleukin 6, Interleukin-6 - genetics, Interleukin-6 - metabolism, Intestinal Mucosa - metabolism, Intestine, Lipopolysaccharides, Lipopolysaccharides - adverse effects, Mice, Mice, Inbred C57BL, Monolayers, Mucin, Nitric oxide, Probiotics, Receptors, Receptors, Aryl Hydrocarbon - genetics, Receptors, Aryl Hydrocarbon - metabolism, Strains (organisms), Tumor Necrosis Factor-alpha - genetics, Tumor Necrosis Factor-alpha - metabolism, Tumor necrosis factor-TNF, Tumor necrosis factor-α, Weight loss, Zonula occludens-1 protein