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A novel isoform of hydroxyacyl-CoA dehydrogenase inhibits cell proliferation
Ist Teil von
Biochemical and biophysical research communications, 2022-05, Vol.606, p.75-79
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
2022
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Hydroxyacyl-CoA dehydrogenase (HADH) catalyzes the third reaction of mitochondrial β-oxidation cascade, while the regulation of its expression and function remains to be elucidated. Using the quantitative translation initiation sequencing (QTI-seq), we have identified that murine Hadh mRNA has two alternative translation start codons. We demonstrated that translation from upstream start codon encodes the mitochondrial isoform of HADH, while translation from downstream start codon produces a short isoform (HADH-S) with predominant nuclear localization. Moreover, overexpression of HADH-S inhibits the proliferation of mouse embryonic fibroblasts. Overall, our results identify a novel isoform of HADH participating in cell proliferation.
•Murine Hadh mRNA has two alternative start codons.•Translation from downstream start codon generates a truncated isoform of HADH.•The truncated isoform of HADH is localized in the nucleus and the cytosol.•The truncated isoform of HADH inhibits MEF cell proliferation.