Details

Autor(en) / Beteiligte

• Wang, Jin-Peng
• Yang, Jian
• Jiao, Peng
• Ren, Qian-Qian
• Luoreng, Zhuo-Ma
• Wang, Xing-Ping
• Ma, Yun
• Wei, Da-Wei

Titel

Differential expression of circRNAs related to lipopolysaccharide-induced inflammation in bovine mammary epithelial cells

Ist Teil von

• Research in veterinary science, 2022-09, Vol.146, p.24-27

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Circular RNAs (circRNAs) are widely involved in inflammatory responses, but their specific regulatory roles in cow mastitis remain controversial. In this study, RNA-seq was used to generate a circRNA expression profile, which identified 71 differentially expressed circRNAs (DEcircRNAs) in lipopolysaccharide (LPS)-stimulated MAC-T bovine mammary epithelial cells (bMECs) at different stages of inflammation. Functional analyses revealed that these DEcircRNAs may be involved in cellular proliferation, apoptosis, migration, and the inflammatory responses through regulation of numerous related signaling pathways. In addition, these data suggest that 2 novel circRNAs, named novel_circ_0004830 and novel_circ_0003097, may act as the key competing endogenous RNAs (ceRNAs) in the regulation of bovine mastitis through binding to inflammation-related microRNAs (miRNAs). These results provide a new angle for the study of the molecular regulatory mechanisms in dairy cow mastitis. •Detection of the differential expression profiles of circRNAs in LPS-induced bovine mammary epithelial cells.•There are 46 up-regulated and 25 down-regulated DEcircRNAs in LPS-induced bovine mammary epithelial cells.•DEcircRNAs may participate in cellular proliferation, apoptosis, migration, and the regulation of bovine mastitis.