Details

Autor(en) / Beteiligte

• Carneiro, M.S.
• Crispim, M.N.
• Wilhelm, C.M.
• Volpato, F.C.Z.
• Barth, A.L.

Titel

Evaluation of filter paper as a means of transporting inactivated Gram‐negative non‐fermentative bacteria and Haemophilus spp. for identification using the MALDI‐TOF MS system

Ist Teil von

• Letters in applied microbiology, 2022-07, Vol.75 (1), p.17-23

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• This study aimed to evaluate the filter paper as a means to transport inactivated Gram‐negative non‐fermentative (GNNF) bacteria and Haemophilus spp. for analysis using MALDI‐TOF MS. A total of 133 isolates were evaluated and the analysis of each isolate was performed directly from original bacterial colony and in filter paper after the processing. To evaluate the agreement between the identification performed directly from the colony and after impregnation in filter paper, we assign the scores: >2·3 as excellent (E); 2·0 to 2·3 as very good (VG); 1·7–1·99 as good (G); <1·7 as unidentified (U). The divergences were classified as: Minor Divergence, Intermediate Divergence and Major Divergence. A total of 80 isolates transported in the filter paper disks presented full category concordance; 39 isolates presented Minor Divergence; 4 isolates present Intermediate Divergence; 4 isolates present Major Divergence and 6 isolates present better results after impregnation in filter paper. The proposed methodology of bacteria transportation presented a sensitivity of 96·9% and a specificity of 100%. The filter paper as a means to transport and storage of inactivated GNNF and Haemophilus spp. may be considered a potential tool for faster, more accurate, biosafe and less‐expensive identification. Significance and Impact of the Study: The study takes care of improvement our understanding about which bacteria can be identified by MALDI‐TOF MS after transport on filter paper. The results will be applicable to favour small laboratories do not have proteomics technology and are unable to carry out the identification at species level of Gram‐negative non‐fermentative (GNNF) bacteria and Haemophilus spp., therefore, need to send isolates that are hardly identified by traditional techniques to reference laboratories. This method of transport is constructive to expansion of identification of GNNF and Haemophilus spp. by MALDI‐TOF MS technology in a safe way since the isolates are transported inactivated.